The purpose of the Uprobe project is to provide the community with an efficient means of experimental access to large-insert cloned DNA (ie, BAC clones) from the full spectrum of vertebrate genomes for which genomic BAC libraries are planned or are currently available. This is being accomplished by the development of "universal" hybridization-based probe resources that can be used to screen BAC libraries from groups of evolutionarily related species (i.e. clades) in order to isolate specific genes/regions of interest for genomic analyses. Toward this end, pre-computed whole-genome sets of universal probes designed for screening genomic libraries from all mammals , subsets of mammals: rodents , carnivores and marsupials , and birds/reptiles can be accessed via database queries (ie, chromosome position, gene name, and/or accession numbers). Custom BED track files for browsing the location of all the probes included in the pre-computed whole-genome probe sets via the UCSC genome browser can be downloaded here . "Universal" probes can also be generated on demand for screening clades of nonhuman primate genomic libraries: Apes & OWM, NWM, Simians, All Primates. In addition, "universal" probes can be custom designed from user provided DNA sequence alignments.
Whole-genome probe sets , related computer programs and experimental protocols can also be directly downloaded from this web site. To read more about the Uprobe project and specific probe sets, click here.
When citing the Uprobe website, please reference:
Kellner et al, Genome Research 15:166-173, 2005 or
Sullivan et al, Nucleic Acids Research 36:W149-W153, 2008.
The Uprobe web site has been made possible by grants from the NIH (R24RR022239 and U01MH068185).